EffiStart™ 2X Probe qPCR Master Mix
EffiStart™ 2X Probe qPCR Master Mix is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of crosscontamination.
Product Note:
• Primer/Probe concentration
Final concentrations of 400 nM (each primer) are suitable for most reactions.To obtain optimal condition, primer concentration can be titrated between 0.2-1 μM.
A final concentration of 200 nM (probe) is suitable for most reactions. To obtain optimal condition, probe concentration can be titrated between 0.1-0.3 μM.
• Annealing/Extension optimization
To obtain optimal condition, annealing/extension temperature can be adjusted between 55°C-65°C, annealing/extension time can be extended up to 60 sec.
• Target length
Appropriate amplicon length should be arranged between 80-200 bp.
Form:
• Primer/Probe concentration
Final concentrations of 400 nM (each primer) are suitable for most reactions.To obtain optimal condition, primer concentration can be titrated between 0.2-1 μM.
A final concentration of 200 nM (probe) is suitable for most reactions. To obtain optimal condition, probe concentration can be titrated between 0.1-0.3 μM.
• Annealing/Extension optimization
To obtain optimal condition, annealing/extension temperature can be adjusted between 55°C-65°C, annealing/extension time can be extended up to 60 sec.
• Target length
Appropriate amplicon length should be arranged between 80-200 bp.
Form:
Liquid
Stability & Storage:
Stability & Storage:
This product is stable after storage at:
• -20°C or -80°C for 12 months under sterile conditions from date of receipt.
Avoid repeated freeze/thaw cycles
Application:
Application:
Reverse transcription, RT-PCR, qPCR
Shipping Conditions:
Blue ice
Shipping Conditions:
Blue ice
